1. ICAR-National Bureau of Plant Genetic Resources, New Delhi, India.

  1. Centre for Development of Advanced Computing, Thiruvananthapuram, Kerala, India.

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Introduction

PGRdup is an R package to facilitate the search for probable/possible duplicate accessions in Plant Genetic Resources (PGR) collections using passport databases. Primarily this package implements a workflow (Fig. 1) designed to fetch groups or sets of germplasm accessions with similar passport data particularly in fields associated with accession names within or across PGR passport databases. It offers a suite of functions for data pre-processing, creation of a searchable Key Word in Context (KWIC) index of keywords associated with accession records and the identification of probable duplicate sets by fuzzy, phonetic and semantic matching of keywords. It also has functions to enable the user to review, modify and validate the probable duplicate sets retrieved.

The goal of this document is to introduce the users to these functions and familiarise them with the workflow intended to fetch probable duplicate sets. This document assumes a basic knowledge of R programming language.

The functions in this package are primarily built using the R packages data.table, igraph, stringdist and stringi.

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Fig. 1. PGRdup workflow and associated functions

Version History

The current version of the package is 0.2.3.9. The previous versions are as follows.

Table 1. Version history of PGRdup R package.

Version Date
0.2 2015-04-14
0.2.1 2015-07-23
0.2.2 2016-03-05
0.2.2.1 2016-03-09
0.2.3 2017-02-01
0.2.3.1 2017-03-15
0.2.3.2 2017-08-05
0.2.3.3 2018-01-13
0.2.3.4 2019-09-19
0.2.3.5 2020-02-10
0.2.3.6 2020-07-27
0.2.3.7 2021-02-17

To know detailed history of changes use news(package='PGRdup').

Installation

The package can be installed using the following functions:

# Install from CRAN
install.packages('PGRdup', dependencies=TRUE)

Uninstalled dependencies (packages which PGRdup depends on viz- data.table, igraph, stringdist and stringi are also installed because of the argument dependencies=TRUE.

Then the package can be loaded using the function

Data Format

The package is essentially designed to operate on PGR passport data present in a data frame object, with each row holding one record and columns representing the attribute fields. For example, consider the dataset GN1000 supplied along with the package.


--------------------------------------------------------------------------------
Welcome to PGRdup version 0.2.3.9


# To know how to use this package type:
  browseVignettes(package = 'PGRdup')
  for the package vignette.

# To know whats new in this version type:
  news(package='PGRdup')
  for the NEWS file.

# To cite the methods in the package type:
  citation(package='PGRdup')

# To suppress this message use:
  suppressPackageStartupMessages(library(PGRdup))
--------------------------------------------------------------------------------
# Load the dataset to the environment
data(GN1000)
# Show the class of the object
class(GN1000)
[1] "data.frame"
# View the first few records in the data frame
head(GN1000)
  CommonName    BotanicalName NationalID                CollNo   DonorID
1  Groundnut Arachis hypogaea   EC100277 Shulamith/ NRCG-14555  ICG-4709
2  Groundnut Arachis hypogaea   EC100280                    NC   ICG5288
3  Groundnut Arachis hypogaea   EC100281               MALIMBA   ICG5289
4  Groundnut Arachis hypogaea   EC100713            EC 100713;   ICG5296
5  Groundnut Arachis hypogaea   EC100715             EC 100715   ICG5298
6  Groundnut Arachis hypogaea   EC100716                        ICG-3150
  OtherID1  OtherID2 BioStatus            SourceCountry TransferYear
1           U4-47-12  Landrace                   Israel         2014
2      NCS      NC 5  Landrace United States of America         2004
3          EC 100281  Landrace                   Malawi         2004
4              STARR  Landrace United States of America         2004
5              COMET  Landrace United States of America         2004
6          ARGENTINE  Landrace United States of America         2014

If the passport data exists as an excel sheet, it can be first converted to a comma-separated values (csv) file or tab delimited file and then easily imported into the R environment using the base functions read.csv and read.table respectively. Similarly read_csv() and read_tsv() from the readr package can also be used. Alternatively, the package readxl can be used to directly read the data from excel. In case of large csv files, the function fread in the data.table package can be used to rapidly load the data.

If the PGR passport data is in a database management system (DBMS), the required table can be imported as a data frame into R. using the appropriate R-database interface package. For example dbConnect for MySQL, ROracle for Oracle etc.

The PGR data downloaded from the genesys database as a Darwin Core - Germplasm zip archive can be imported into the R environment as a flat file data.frame using the read.genesys function.

# Import the DwC-Germplasm zip archive "genesys-accessions-filtered.zip"
PGRgenesys <- read.genesys("genesys-accessions-filtered.zip",
                           scrub.names.space = TRUE, readme = TRUE)

Data Pre-processing

Data pre-processing is a critical step which can affect the quality of the probable duplicate sets being retrieved. It involves data standardization as well as data cleaning which can be achieved using the functions DataClean, MergeKW, MergePrefix and MergeSuffix.

DataClean function can be used to clean the character strings in passport data fields(columns) specified as the input character vector x according to the conditions specified in the arguments.

Commas, semicolons and colons which are sometimes used to separate multiple strings or names within the same field can be replaced with a single space using the logical arguments fix.comma, fix.semcol and fix.col respectively.

x <- c("A 14; EC 1697", "U 4-4-28; EC 21078; A 32", "PI 262801:CIAT 9075:GKP 9553/90",
       "NCAC 16049, PI 261987, RCM 493-3")
x
[1] "A 14; EC 1697"                    "U 4-4-28; EC 21078; A 32"        
[3] "PI 262801:CIAT 9075:GKP 9553/90"  "NCAC 16049, PI 261987, RCM 493-3"
# Replace ',', ':' and ';' with space
DataClean(x, fix.comma=TRUE, fix.semcol=TRUE, fix.col=TRUE,
          fix.bracket=FALSE, fix.punct=FALSE, fix.space=FALSE, fix.sep=FALSE,
          fix.leadzero=FALSE)
[1] "A 14  EC 1697"                    "U 4-4-28  EC 21078  A 32"        
[3] "PI 262801 CIAT 9075 GKP 9553/90"  "NCAC 16049  PI 261987  RCM 493-3"

Similarly the logical argument fix.bracket can be used to replace all brackets including parenthesis, square brackets and curly brackets with space.

x <- c("(NRCG-1738)/(NFG649)", "26-5-1[NRCG-2528]", "Ah 1182 {NRCG-4340}")
x
[1] "(NRCG-1738)/(NFG649)" "26-5-1[NRCG-2528]"    "Ah 1182 {NRCG-4340}" 
# Replace parenthesis, square brackets and curly brackets with space
DataClean(x, fix.comma=FALSE, fix.semcol=FALSE, fix.col=FALSE,
          fix.bracket=TRUE,
          fix.punct=FALSE, fix.space=FALSE, fix.sep=FALSE, fix.leadzero=FALSE)
[1] "NRCG-1738 / NFG649" "26-5-1 NRCG-2528"   "AH 1182  NRCG-4340"

The logical argument fix.punct can be used to remove all punctuation from the data.

x <- c("#26-6-3-1", "Culture No. 857", "U/4/47/13")
x
[1] "#26-6-3-1"       "Culture No. 857" "U/4/47/13"      
# Remove punctuation
DataClean(x, fix.comma=FALSE, fix.semcol=FALSE, fix.col=FALSE, fix.bracket=FALSE,
          fix.punct=TRUE,
          fix.space=FALSE, fix.sep=FALSE, fix.leadzero=FALSE)
[1] "26631"          "CULTURE NO 857" "U44713"        

fix.space can be used to convert all space characters such as tab, newline, vertical tab, form feed and carriage return to spaces and finally convert multiple spaces to single space.

x <- c("RS   1", "GKSPScGb 208  PI 475855")
x
[1] "RS   1"                  "GKSPScGb 208  PI 475855"
# Replace all space characters to space and convert multiple spaces to single space
DataClean(x, fix.comma=FALSE, fix.semcol=FALSE, fix.col=FALSE,
          fix.bracket=FALSE, fix.punct=FALSE,
          fix.space=TRUE,
          fix.sep=FALSE, fix.leadzero=FALSE)
[1] "RS 1"                   "GKSPSCGB 208 PI 475855"

fix.sep can be used to merge together accession identifiers composed of alphabetic characters separated from a series of digits by a space character.

x <- c("NCAC 18078", "AH 6481", "ICG 2791")
x
[1] "NCAC 18078" "AH 6481"    "ICG 2791"  
# Merge alphabetic character separated from a series of digits by a space
DataClean(x, fix.comma=FALSE, fix.semcol=FALSE, fix.col=FALSE,
          fix.bracket=FALSE, fix.punct=FALSE, fix.space=FALSE,
          fix.sep=TRUE,
          fix.leadzero=FALSE)
[1] "NCAC18078" "AH6481"    "ICG2791"  

fix.leadzero can be used to remove leading zeros from accession name fields to facilitate matching to identify probable duplicates.

x <- c("EC 0016664", "EC0001690")
x
[1] "EC 0016664" "EC0001690" 
# Remove leading zeros
DataClean(x, fix.comma=FALSE, fix.semcol=FALSE, fix.col=FALSE,
          fix.bracket=FALSE, fix.punct=FALSE, fix.space=FALSE, fix.sep=FALSE,
          fix.leadzero=TRUE)
[1] "EC 16664" "EC1690"  

This function can hence be made use of in tidying up multiple forms of messy data existing in fields associated with accession names in PGR passport databases (Table 1).

names <- c("S7-12-6", "ICG-3505", "U 4-47-18;EC 21127", "AH 6481", "RS   1",
           "AK 12-24", "2-5 (NRCG-4053)", "T78, Mwitunde", "ICG 3410",
           "#648-4 (Gwalior)", "TG4;U/4/47/13", "EC0021003")
names
 [1] "S7-12-6"            "ICG-3505"           "U 4-47-18;EC 21127"
 [4] "AH 6481"            "RS   1"             "AK 12-24"          
 [7] "2-5 (NRCG-4053)"    "T78, Mwitunde"      "ICG 3410"          
[10] "#648-4 (Gwalior)"   "TG4;U/4/47/13"      "EC0021003"         
# Clean the data
DataClean(names)
 [1] "S7126"          "ICG3505"        "U44718 EC21127" "AH6481"        
 [5] "RS1"            "AK1224"         "25 NRCG4053"    "T78 MWITUNDE"  
 [9] "ICG3410"        "6484 GWALIOR"   "TG4 U44713"     "EC21003"       

Table 2. Data pre-processing using DataClean.

names DataClean(names)
S7-12-6 S7126
ICG-3505 ICG3505
U 4-47-18;EC 21127 U44718 EC21127
AH 6481 AH6481
RS 1 RS1
AK 12-24 AK1224
2-5 (NRCG-4053) 25 NRCG4053
T78, Mwitunde T78 MWITUNDE
ICG 3410 ICG3410
#648-4 (Gwalior) 6484 GWALIOR
TG4;U/4/47/13 TG4 U44713
EC0021003 EC21003

Several common keyword string pairs or keyword prefixes and suffixes exist in fields associated with accession names in PGR passport databases. They can be merged using the functions MergeKW, MergePrefix and MergeSuffix respectively. The keyword string pairs, prefixes and suffixes can be supplied as a list or a vector to the argument y in these functions.

names <- c("Punjab Bold", "Gujarat- Dwarf", "Nagpur.local", "SAM COL 144",
           "SAM COL--280", "NIZAMABAD-LOCAL", "Dark Green Mutant",
           "Dixie-Giant", "Georgia- Bunch", "Uganda-erect", "Small Japan",
           "Castle  Cary", "Punjab erect", "Improved small japan",
           "Dark Purple")
names
 [1] "Punjab Bold"          "Gujarat- Dwarf"       "Nagpur.local"        
 [4] "SAM COL 144"          "SAM COL--280"         "NIZAMABAD-LOCAL"     
 [7] "Dark Green Mutant"    "Dixie-Giant"          "Georgia- Bunch"      
[10] "Uganda-erect"         "Small Japan"          "Castle  Cary"        
[13] "Punjab erect"         "Improved small japan" "Dark Purple"         
# Merge pairs of strings
y1 <- list(c("Gujarat", "Dwarf"), c("Castle", "Cary"), c("Small", "Japan"),
           c("Big", "Japan"), c("Mani", "Blanco"), c("Uganda", "Erect"),
           c("Mota", "Company"))
names <- MergeKW(names, y1, delim = c("space", "dash", "period"))

# Merge prefix strings
y2 <- c("Light", "Small", "Improved", "Punjab", "SAM", "Dark")
names <- MergePrefix(names, y2, delim = c("space", "dash", "period"))

# Merge suffix strings
y3 <- c("Local", "Bold", "Cary", "Mutant", "Runner", "Giant", "No.",
        "Bunch", "Peanut")
names <- MergeSuffix(names, y3, delim = c("space", "dash", "period"))

names
 [1] "PunjabBold"         "GujaratDwarf"       "Nagpurlocal"       
 [4] "SAMCOL 144"         "SAMCOL--280"        "NIZAMABADLOCAL"    
 [7] "DarkGreenMutant"    "DixieGiant"         "GeorgiaBunch"      
[10] "Ugandaerect"        "SmallJapan"         "CastleCary"        
[13] "Punjaberect"        "Improvedsmalljapan" "DarkPurple"        

These functions can be applied over multiple columns(fields) in a data frame using the lapply function.

# Load example dataset
GN <- GN1000

# Specify as a vector the database fields to be used
GNfields <- c("NationalID", "CollNo", "DonorID", "OtherID1", "OtherID2")
head(GN[GNfields])
  NationalID                CollNo   DonorID OtherID1  OtherID2
1   EC100277 Shulamith/ NRCG-14555  ICG-4709           U4-47-12
2   EC100280                    NC   ICG5288      NCS      NC 5
3   EC100281               MALIMBA   ICG5289          EC 100281
4   EC100713            EC 100713;   ICG5296              STARR
5   EC100715             EC 100715   ICG5298              COMET
6   EC100716                        ICG-3150          ARGENTINE
# Clean the data
GN[GNfields] <- lapply(GN[GNfields], function(x) DataClean(x))
y1 <- list(c("Gujarat", "Dwarf"), c("Castle", "Cary"), c("Small", "Japan"),
c("Big", "Japan"), c("Mani", "Blanco"), c("Uganda", "Erect"),
c("Mota", "Company"))
y2 <- c("Dark", "Light", "Small", "Improved", "Punjab", "SAM")
y3 <- c("Local", "Bold", "Cary", "Mutant", "Runner", "Giant", "No.",
        "Bunch", "Peanut")
GN[GNfields] <- lapply(GN[GNfields],
                       function(x) MergeKW(x, y1, delim = c("space", "dash")))
GN[GNfields] <- lapply(GN[GNfields],
                       function(x) MergePrefix(x, y2, delim = c("space", "dash")))
GN[GNfields] <- lapply(GN[GNfields],
                       function(x) MergeSuffix(x, y3, delim = c("space", "dash")))
head(GN[GNfields])
  NationalID              CollNo DonorID OtherID1  OtherID2
1   EC100277 SHULAMITH NRCG14555 ICG4709             U44712
2   EC100280                  NC ICG5288      NCS       NC5
3   EC100281             MALIMBA ICG5289           EC100281
4   EC100713            EC100713 ICG5296              STARR
5   EC100715            EC100715 ICG5298              COMET
6   EC100716                     ICG3150          ARGENTINE

Generation of KWIC Index

The function KWIC generates a Key Word in Context index (Knüpffer 1988; Knüpffer, Frese, and Jongen 1997) from the data frame of a PGR passport database based on the fields(columns) specified in the argument fields along with the keyword frequencies and gives the output as a list of class KWIC. The first element of the vector specified in fields is considered as the primary key or identifier which uniquely identifies all rows in the data frame.

This function fetches keywords from different fields specified, which can be subsequently used for matching to identify probable duplicates. The frequencies of the keywords retrieved can help in determining if further data pre-processing is required and also to decide whether any common keywords can be exempted from matching (Fig. 2).

# Load example dataset
GN <- GN1000

# Specify as a vector the database fields to be used
GNfields <- c("NationalID", "CollNo", "DonorID", "OtherID1", "OtherID2")

# Clean the data
GN[GNfields] <- lapply(GN[GNfields], function(x) DataClean(x))
y1 <- list(c("Gujarat", "Dwarf"), c("Castle", "Cary"), c("Small", "Japan"),
c("Big", "Japan"), c("Mani", "Blanco"), c("Uganda", "Erect"),
c("Mota", "Company"))
y2 <- c("Dark", "Light", "Small", "Improved", "Punjab", "SAM")
y3 <- c("Local", "Bold", "Cary", "Mutant", "Runner", "Giant", "No.",
        "Bunch", "Peanut")
GN[GNfields] <- lapply(GN[GNfields],
                       function(x) MergeKW(x, y1, delim = c("space", "dash")))
GN[GNfields] <- lapply(GN[GNfields],
                       function(x) MergePrefix(x, y2, delim = c("space", "dash")))
GN[GNfields] <- lapply(GN[GNfields],
                       function(x) MergeSuffix(x, y3, delim = c("space", "dash")))

# Generate the KWIC index
GNKWIC <- KWIC(GN, GNfields, min.freq = 1)
class(GNKWIC)
[1] "KWIC"
GNKWIC
KWIC fields : NationalID CollNo DonorID OtherID1 OtherID2
Number of keywords : 3893
Number of distinct keywords : 3109
# Retrieve the KWIC index from the KWIC object
KWIC <- GNKWIC[[1]]
KWIC <- KWIC[order(KWIC$KEYWORD, decreasing = TRUE),]
head(KWIC[,c("PRIM_ID", "KWIC_L", "KWIC_KW", "KWIC_R")], n = 10)
      PRIM_ID                                     KWIC_L  KWIC_KW
550  EC490380            EC490380 =  = ICG1122 =  = LIN      YUCH
435   EC36893                                 EC36893 =      YUAN
434   EC36893                            EC36893 = YUAN     YOUNG
1287 EC613524       EC613524 = NRCG9225 =  = PEI KANGPE    YOUDON
1703 IC113088                       IC113088 =  =  = SB        XI
1741 IC296965 IC296965 = SB X11 X V11 = ICG1769 =  = SB        XI
3385 IC445197                                IC445197 =   X144B28
3483 IC494754                IC494754 =  = ICG7686 =  =   X144B28
2090 IC304018    IC304018 = 144B19B NRCG = ICG1561 =  =  X144B19B
1735 IC296965                             IC296965 = SB       X11
                                KWIC_R
550                               TSAO
435   YOUNG TOU = ICG5241 =  = EC36893
434         TOU = ICG5241 =  = EC36893
1287                                 =
1703                        = IC305003
1741                             X VII
3385           B = ICG2113 =  = LIMDI4
3483                                 B
2090                                  
1735  X V11 = ICG1769 =  = SB XI X VII
# Retrieve the keyword frequencies from the KWIC object
KeywordFreq <- GNKWIC[[2]]
head(KeywordFreq)
  Keyword Freq
1   OVERO   25
2      S1   19
3       A   11
4     RED   11
5    OVER   10
6  PURPLE   10

Fig. 2. Word cloud of keywords retrieved

The function will throw an error in case of duplicates or NULL values in the primary key/ID field mentioned.

GN <- GN1000
GN[GNfields] <- lapply(GN[GNfields], function(x) DataClean(x))
# Generate dummy duplicates for illustration
GN[1001:1005,] <- GN[1:5,]
# Generate dummy NULL values for illustration
GN[1001,3] <- ""
GN[1002,3] <- ""
GN[1001:1005,]
     CommonName    BotanicalName NationalID              CollNo DonorID
1001  Groundnut Arachis hypogaea            SHULAMITH NRCG14555 ICG4709
1002  Groundnut Arachis hypogaea                             NC ICG5288
1003  Groundnut Arachis hypogaea   EC100281             MALIMBA ICG5289
1004  Groundnut Arachis hypogaea   EC100713            EC100713 ICG5296
1005  Groundnut Arachis hypogaea   EC100715            EC100715 ICG5298
     OtherID1 OtherID2 BioStatus            SourceCountry TransferYear
1001            U44712  Landrace                   Israel         2014
1002      NCS      NC5  Landrace United States of America         2004
1003          EC100281  Landrace                   Malawi         2004
1004             STARR  Landrace United States of America         2004
1005             COMET  Landrace United States of America         2004
GNKWIC <- KWIC(GN, GNfields, min.freq=1)
Error in KWIC(GN, GNfields, min.freq = 1) :
  Primary key/ID field should be unique and not NULL
 Use PGRdup::ValidatePrimKey() to identify and rectify the aberrant records first

The erroneous records can be identified using the helper function ValidatePrimKey.

# Validate the primary key/ID field for duplication or existence of NULL values
ValidatePrimKey(x = GN, prim.key = "NationalID")
$message1
[1] "ERROR: Duplicated records found in prim.key field"

$Duplicates
     CommonName    BotanicalName NationalID              CollNo DonorID
1001  Groundnut Arachis hypogaea            SHULAMITH NRCG14555 ICG4709
1002  Groundnut Arachis hypogaea                             NC ICG5288
3     Groundnut Arachis hypogaea   EC100281             MALIMBA ICG5289
1003  Groundnut Arachis hypogaea   EC100281             MALIMBA ICG5289
4     Groundnut Arachis hypogaea   EC100713            EC100713 ICG5296
1004  Groundnut Arachis hypogaea   EC100713            EC100713 ICG5296
5     Groundnut Arachis hypogaea   EC100715            EC100715 ICG5298
1005  Groundnut Arachis hypogaea   EC100715            EC100715 ICG5298
     OtherID1 OtherID2 BioStatus            SourceCountry TransferYear
1001            U44712  Landrace                   Israel         2014
1002      NCS      NC5  Landrace United States of America         2004
3             EC100281  Landrace                   Malawi         2004
1003          EC100281  Landrace                   Malawi         2004
4                STARR  Landrace United States of America         2004
1004             STARR  Landrace United States of America         2004
5                COMET  Landrace United States of America         2004
1005             COMET  Landrace United States of America         2004

$message2
[1] "ERROR: NULL records found in prim.key field"

$NullRecords
     CommonName    BotanicalName NationalID              CollNo DonorID
1001  Groundnut Arachis hypogaea            SHULAMITH NRCG14555 ICG4709
1002  Groundnut Arachis hypogaea                             NC ICG5288
     OtherID1 OtherID2 BioStatus            SourceCountry TransferYear primdup
1001            U44712  Landrace                   Israel         2014    TRUE
1002      NCS      NC5  Landrace United States of America         2004    TRUE
# Remove the offending records
GN <- GN[-c(1001:1005), ]
# Validate again
ValidatePrimKey(x = GN, prim.key = "NationalID")
$message1
[1] "OK: No duplicated records found in prim.key field"

$Duplicates
NULL

$message2
[1] "OK: No NULL records found in prim.key field"

$NullRecords
NULL

Retrieval of Probable Duplicate Sets

Once KWIC indexes are generated, probable duplicates of germplasm accessions can be identified by fuzzy, phonetic and semantic matching of the associated keywords using the function ProbDup. The sets are retrieved as a list of data frames of class ProbDup.

Keywords that are not to be used for matching can be specified as a vector in the excep argument.

Methods

The function can execute matching according to either one of the following three methods as specified by the method argument.

  1. Method "a" : Performs string matching of keywords in a single KWIC index to identify probable duplicates of accessions in a single PGR passport database.
# Load example dataset
GN <- GN1000

# Specify as a vector the database fields to be used
GNfields <- c("NationalID", "CollNo", "DonorID", "OtherID1", "OtherID2")

# Clean the data
GN[GNfields] <- lapply(GN[GNfields], function(x) DataClean(x))
y1 <- list(c("Gujarat", "Dwarf"), c("Castle", "Cary"), c("Small", "Japan"),
c("Big", "Japan"), c("Mani", "Blanco"), c("Uganda", "Erect"),
c("Mota", "Company"))
y2 <- c("Dark", "Light", "Small", "Improved", "Punjab", "SAM")
y3 <- c("Local", "Bold", "Cary", "Mutant", "Runner", "Giant", "No.",
        "Bunch", "Peanut")
GN[GNfields] <- lapply(GN[GNfields],
                       function(x) MergeKW(x, y1, delim = c("space", "dash")))
GN[GNfields] <- lapply(GN[GNfields],
                       function(x) MergePrefix(x, y2, delim = c("space", "dash")))
GN[GNfields] <- lapply(GN[GNfields],
                       function(x) MergeSuffix(x, y3, delim = c("space", "dash")))

# Generate the KWIC index
GNKWIC <- KWIC(GN, GNfields)
# Specify the exceptions as a vector
exep <- c("A", "B", "BIG", "BOLD", "BUNCH", "C", "COMPANY", "CULTURE",
         "DARK", "E", "EARLY", "EC", "ERECT", "EXOTIC", "FLESH", "GROUNDNUT",
         "GUTHUKAI", "IMPROVED", "K", "KUTHUKADAL", "KUTHUKAI", "LARGE",
         "LIGHT", "LOCAL", "OF", "OVERO", "P", "PEANUT", "PURPLE", "R",
         "RED", "RUNNER", "S1", "SAM", "SMALL", "SPANISH", "TAN", "TYPE",
         "U", "VALENCIA", "VIRGINIA", "WHITE")

# Fetch fuzzy duplicates by method 'a'
GNdup <- ProbDup(kwic1 = GNKWIC, method = "a", excep = exep, fuzzy = TRUE,
                 phonetic = FALSE, semantic = FALSE)
Fuzzy matching

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class(GNdup)
[1] "ProbDup"
GNdup
Method : a

KWIC1 fields : NationalID CollNo DonorID OtherID1 OtherID2
 
                No..of.Sets    No..of.Records
FuzzyDuplicates         378               745
Total                   378 745(Distinct:745)
# Fetch phonetic duplicates by method 'a'
GNdup <- ProbDup(kwic1 = GNKWIC, method = "a", excep = exep, fuzzy = FALSE,
                 phonetic = TRUE, semantic = FALSE)
Phonetic matching

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class(GNdup)
[1] "ProbDup"
GNdup
Method : a

KWIC1 fields : NationalID CollNo DonorID OtherID1 OtherID2
 
                   No..of.Sets    No..of.Records
PhoneticDuplicates          99               260
Total                       99 260(Distinct:260)
  1. Method "b" : Performs string matching of keywords in the first KWIC index (query) with that of the keywords in the second index (source) to identify probable duplicates of accessions of the first PGR passport database among the accessions in the second database.

  2. Method "c" : Performs string matching of keywords in two different KWIC indexes jointly to identify probable duplicates of accessions from among two PGR passport databases.

# Load PGR passport databases
GN1 <- GN1000[!grepl("^ICG", GN1000$DonorID), ]
GN1$DonorID <- NULL
GN2 <- GN1000[grepl("^ICG", GN1000$DonorID), ]
GN2$NationalID <- NULL

# Specify database fields to use
GN1fields <- c("NationalID", "CollNo", "OtherID1", "OtherID2")
GN2fields <- c("DonorID", "CollNo", "OtherID1", "OtherID2")

# Clean the data
GN1[GN1fields] <- lapply(GN1[GN1fields], function(x) DataClean(x))
GN2[GN2fields] <- lapply(GN2[GN2fields], function(x) DataClean(x))
y1 <- list(c("Gujarat", "Dwarf"), c("Castle", "Cary"), c("Small", "Japan"),
c("Big", "Japan"), c("Mani", "Blanco"), c("Uganda", "Erect"),
c("Mota", "Company"))
y2 <- c("Dark", "Light", "Small", "Improved", "Punjab", "SAM")
y3 <- c("Local", "Bold", "Cary", "Mutant", "Runner", "Giant", "No.",
        "Bunch", "Peanut")
GN1[GN1fields] <- lapply(GN1[GN1fields],
                         function(x) MergeKW(x, y1, delim = c("space", "dash")))
GN1[GN1fields] <- lapply(GN1[GN1fields],
                         function(x) MergePrefix(x, y2, delim = c("space", "dash")))
GN1[GN1fields] <- lapply(GN1[GN1fields],
                         function(x) MergeSuffix(x, y3, delim = c("space", "dash")))
GN2[GN2fields] <- lapply(GN2[GN2fields],
                         function(x) MergeKW(x, y1, delim = c("space", "dash")))
GN2[GN2fields] <- lapply(GN2[GN2fields],
                         function(x) MergePrefix(x, y2, delim = c("space", "dash")))
GN2[GN2fields] <- lapply(GN2[GN2fields],
                         function(x) MergeSuffix(x, y3, delim = c("space", "dash")))

# Remove duplicated DonorID records in GN2
GN2 <- GN2[!duplicated(GN2$DonorID), ]

# Generate KWIC index
GN1KWIC <- KWIC(GN1, GN1fields)
GN2KWIC <- KWIC(GN2, GN2fields)

# Specify the exceptions as a vector
exep <- c("A", "B", "BIG", "BOLD", "BUNCH", "C", "COMPANY", "CULTURE",
         "DARK", "E", "EARLY", "EC", "ERECT", "EXOTIC", "FLESH", "GROUNDNUT",
         "GUTHUKAI", "IMPROVED", "K", "KUTHUKADAL", "KUTHUKAI", "LARGE",
         "LIGHT", "LOCAL", "OF", "OVERO", "P", "PEANUT", "PURPLE", "R",
         "RED", "RUNNER", "S1", "SAM", "SMALL", "SPANISH", "TAN", "TYPE",
         "U", "VALENCIA", "VIRGINIA", "WHITE")

# Fetch fuzzy and phonetic duplicate sets by method b
GNdupb <- ProbDup(kwic1 = GN1KWIC, kwic2 = GN2KWIC, method = "b",
                  excep = exep, fuzzy = TRUE, phonetic = TRUE,
                  encoding = "primary", semantic = FALSE)
Fuzzy matching

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Phonetic matching

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class(GNdupb)
[1] "ProbDup"
GNdupb
Method : b

KWIC1 fields : NationalID CollNo OtherID1 OtherID2

KWIC2 fields : DonorID CollNo OtherID1 OtherID2
 
                   No..of.Sets    No..of.Records
FuzzyDuplicates            107               353
PhoneticDuplicates          41               126
Total                      148 479(Distinct:383)
# Fetch fuzzy and phonetic duplicate sets by method c
GNdupc <- ProbDup(kwic1 = GN1KWIC, kwic2 = GN2KWIC, method = "c",
                  excep = exep, fuzzy = TRUE, phonetic = TRUE,
                  encoding = "primary", semantic = FALSE)
Fuzzy matching

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Phonetic matching

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class(GNdupc)
[1] "ProbDup"
GNdupc
Method : c

KWIC1 fields : NationalID CollNo OtherID1 OtherID2

KWIC2 fields : DonorID CollNo OtherID1 OtherID2
 
                   No..of.Sets    No..of.Records
FuzzyDuplicates            363               724
PhoneticDuplicates          98               257
Total                      461 981(Distinct:741)

Matching Strategies

  1. Fuzzy matching or approximate string matching of keywords is carried out by computing the generalized levenshtein (edit) distance between them. This distance measure counts the number of deletions, insertions and substitutions necessary to turn one string to another.
# Load example dataset
GN <- GN1000

# Specify as a vector the database fields to be used
GNfields <- c("NationalID", "CollNo", "DonorID", "OtherID1", "OtherID2")

# Clean the data
GN[GNfields] <- lapply(GN[GNfields], function(x) DataClean(x))
y1 <- list(c("Gujarat", "Dwarf"), c("Castle", "Cary"), c("Small", "Japan"),
c("Big", "Japan"), c("Mani", "Blanco"), c("Uganda", "Erect"),
c("Mota", "Company"))
y2 <- c("Dark", "Light", "Small", "Improved", "Punjab", "SAM")
y3 <- c("Local", "Bold", "Cary", "Mutant", "Runner", "Giant", "No.",
        "Bunch", "Peanut")
GN[GNfields] <- lapply(GN[GNfields],
                       function(x) MergeKW(x, y1, delim = c("space", "dash")))
GN[GNfields] <- lapply(GN[GNfields],
                       function(x) MergePrefix(x, y2, delim = c("space", "dash")))
GN[GNfields] <- lapply(GN[GNfields],
                       function(x) MergeSuffix(x, y3, delim = c("space", "dash")))

# Generate the KWIC index
GNKWIC <- KWIC(GN, GNfields)

# Specify the exceptions as a vector
exep <- c("A", "B", "BIG", "BOLD", "BUNCH", "C", "COMPANY", "CULTURE",
         "DARK", "E", "EARLY", "EC", "ERECT", "EXOTIC", "FLESH", "GROUNDNUT",
         "GUTHUKAI", "IMPROVED", "K", "KUTHUKADAL", "KUTHUKAI", "LARGE",
         "LIGHT", "LOCAL", "OF", "OVERO", "P", "PEANUT", "PURPLE", "R",
         "RED", "RUNNER", "S1", "SAM", "SMALL", "SPANISH", "TAN", "TYPE",
         "U", "VALENCIA", "VIRGINIA", "WHITE")

# Fetch fuzzy duplicates
GNdup <- ProbDup(kwic1 = GNKWIC, method = "a", excep = exep, 
                 fuzzy = TRUE, max.dist = 3,
                 phonetic = FALSE, semantic = FALSE)
Fuzzy matching

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GNdup
Method : a

KWIC1 fields : NationalID CollNo DonorID OtherID1 OtherID2
 
                No..of.Sets    No..of.Records
FuzzyDuplicates         378               745
Total                   378 745(Distinct:745)

The maximum distance to be considered for a match can be specified by max.dist argument.

GNdup <- ProbDup(kwic1 = GNKWIC, method = "a", excep = exep, 
                 fuzzy = TRUE, max.dist = 1,
                 phonetic = FALSE, semantic = FALSE)
Fuzzy matching

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GNdup
Method : a

KWIC1 fields : NationalID CollNo DonorID OtherID1 OtherID2
 
                No..of.Sets    No..of.Records
FuzzyDuplicates         288               679
Total                   288 679(Distinct:679)

Exact matching can be enforced with the argument force.exact set as TRUE. It can be used to avoid fuzzy matching when the number of alphabet characters in keywords is lesser than a critical value (max.alpha). Similarly, the value of max.digit can also be set according to the requirements to enforce exact matching. The default value of Inf avoids fuzzy matching and enforces exact matching for all keywords having any numerical characters. If max.digit and max.alpha are both set to Inf, exact matching will be enforced for all the keywords.

When exact matching is enforced, for keywords having both alphabet and numeric characters and with the number of alphabet characters greater than max.digit, matching will be carried out separately for alphabet and numeric characters present.

GNdup <- ProbDup(kwic1 = GNKWIC, method = "a", excep = exep, 
                 fuzzy = TRUE, force.exact = TRUE, max.alpha = 4, max.digit = Inf,
                 phonetic = FALSE, semantic = FALSE)
Fuzzy matching

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GNdup
Method : a

KWIC1 fields : NationalID CollNo DonorID OtherID1 OtherID2
 
                No..of.Sets    No..of.Records
FuzzyDuplicates         378               745
Total                   378 745(Distinct:745)
  1. Phonetic matching of keywords is carried out using the Double Metaphone phonetic algorithm which is implemented as the helper function DoubleMetaphone, (Philips 2000), to identify keywords that have the similar pronunciation.
GNdup <- ProbDup(kwic1 = GNKWIC, method = "a", excep = exep, 
                 fuzzy = FALSE,
                 phonetic = TRUE,
                 semantic = FALSE)
Phonetic matching

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GNdup
Method : a

KWIC1 fields : NationalID CollNo DonorID OtherID1 OtherID2
 
                   No..of.Sets    No..of.Records
PhoneticDuplicates          99               260
Total                       99 260(Distinct:260)

Either the primary or alternate encodings can be used by specifying the encoding argument.

GNdup <- ProbDup(kwic1 = GNKWIC, method = "a", excep = exep, 
                 fuzzy = FALSE,
                 phonetic = TRUE, encoding = "alternate",
                 semantic = FALSE)
Phonetic matching

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GNdup
Method : a

KWIC1 fields : NationalID CollNo DonorID OtherID1 OtherID2
 
                   No..of.Sets    No..of.Records
PhoneticDuplicates          98               263
Total                       98 263(Distinct:263)

The argument phon.min.alpha sets the limits for the number of alphabet characters to be present in a string for executing phonetic matching.

GNdup <- ProbDup(kwic1 = GNKWIC, method = "a", excep = exep, 
                 fuzzy = FALSE,
                 phonetic = TRUE, encoding = "alternate", phon.min.alpha = 4,
                 semantic = FALSE)
Phonetic matching

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GNdup
Method : a

KWIC1 fields : NationalID CollNo DonorID OtherID1 OtherID2
 
                   No..of.Sets    No..of.Records
PhoneticDuplicates         304               451
Total                      304 451(Distinct:451)

Similarly min.enc sets the limits for the number of characters to be present in the encoding of a keyword for phonetic matching.

GNdup <- ProbDup(kwic1 = GNKWIC, method = "a", excep = exep, 
                 fuzzy = FALSE,
                 phonetic = TRUE, encoding = "alternate", min.enc = 4,
                 semantic = FALSE)
Phonetic matching

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GNdup
Method : a

KWIC1 fields : NationalID CollNo DonorID OtherID1 OtherID2
 
                   No..of.Sets    No..of.Records
PhoneticDuplicates          59               156
Total                       59 156(Distinct:156)
  1. Semantic matching matches keywords based on a list of accession name synonyms supplied as list with character vectors of synonym sets (synsets) to the syn argument. Synonyms in this context refer to interchangeable identifiers or names by which an accession is recognized. Multiple keywords specified as members of the same synset in syn are matched. To facilitate accurate identification of synonyms from the KWIC index, identical data standardization operations using the Merge* and DataClean functions for both the original database fields and the synset list are recommended.
# Specify the synsets as a list
syn <- list(c("CHANDRA", "AH 114"), c("TG-1", "VIKRAM"))

# Clean the data in the synsets
syn <- lapply(syn, DataClean)

GNdup <- ProbDup(kwic1 = GNKWIC, method = "a", excep = exep, 
                 fuzzy = FALSE, phonetic = FALSE,
                 semantic = TRUE, syn = syn)
Semantic matching

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GNdup
Method : a

KWIC1 fields : NationalID CollNo DonorID OtherID1 OtherID2
 
                   No..of.Sets No..of.Records
SemanticDuplicates           2              5
Total                        2  5(Distinct:5)

Memory and Speed Constraints

As the number of keywords in the KWIC indexes increases, the memory consumption by the function also increases proportionally. This is due to the reason that for string matching, this function relies upon creation of a n\(\times\)m matrix of all possible keyword pairs for comparison, where n and m are the number of keywords in the query and source indexes respectively. This can lead to cannot allocate vector of size... errors in case of large KWIC indexes where the comparison matrix is too large to reside in memory. In such a case, the chunksize argument can be reduced from the default 1000 to get the appropriate size of the KWIC index keyword block to be used for searching for matches at a time. However a smaller chunksize may lead to longer computation time due to the memory-time trade-off.

The progress of matching is displayed in the console as number of keyword blocks completed out of the total number of blocks, the percentage of achievement and a text-based progress bar.

In case of multi-byte characters in keywords, the speed of keyword matching is further dependent upon the useBytes argument as described in help("stringdist-encoding") for the stringdist function in the namesake package (Loo 2014), which is made use of here for string matching.

The CPU time taken for retrieval of probable duplicate sets under different options for the arguments chunksize and useBytes can be visualized using the microbenchmark package (Fig. 3).

# Load example dataset
GN <- GN1000

# Specify as a vector the database fields to be used
GNfields <- c("NationalID", "CollNo", "DonorID", "OtherID1", "OtherID2")

# Clean the data
GN[GNfields] <- lapply(GN[GNfields], function(x) DataClean(x))
y1 <- list(c("Gujarat", "Dwarf"), c("Castle", "Cary"), c("Small", "Japan"),
           c("Big", "Japan"), c("Mani", "Blanco"), c("Uganda", "Erect"),
           c("Mota", "Company"))
y2 <- c("Dark", "Light", "Small", "Improved", "Punjab", "SAM")
y3 <- c("Local", "Bold", "Cary", "Mutant", "Runner", "Giant", "No.", "Bunch", "Peanut")
GN[GNfields] <- lapply(GN[GNfields],
                       function(x) MergeKW(x, y1, delim = c("space", "dash")))
GN[GNfields] <- lapply(GN[GNfields],
                       function(x) MergePrefix(x, y2, delim = c("space", "dash")))
GN[GNfields] <- lapply(GN[GNfields],
                       function(x) MergeSuffix(x, y3, delim = c("space", "dash")))

# Generate the KWIC index
GNKWIC <- KWIC(GN, GNfields)

# Specify the exceptions as a vector
exep <- c("A", "B", "BIG", "BOLD", "BUNCH", "C", "COMPANY", "CULTURE", "DARK",
          "E", "EARLY", "EC", "ERECT", "EXOTIC", "FLESH", "GROUNDNUT", "GUTHUKAI",
          "IMPROVED", "K", "KUTHUKADAL", "KUTHUKAI", "LARGE", "LIGHT", "LOCAL",
          "OF", "OVERO", "P", "PEANUT", "PURPLE", "R", "RED", "RUNNER", "S1", "SAM",
          "SMALL", "SPANISH", "TAN", "TYPE", "U", "VALENCIA", "VIRGINIA", "WHITE")

# Specify the synsets as a list
syn <- list(c("CHANDRA", "AH 114"), c("TG-1", "VIKRAM"))
syn <- lapply(syn, DataClean)
timings <- microbenchmark::microbenchmark(
  # Fetch duplicate sets with default chunk.size
  t1 = ProbDup(kwic1 = GNKWIC, method = "a", excep = exep,
                                     chunksize = 1000, useBytes = TRUE,
                                     fuzzy = TRUE, phonetic = TRUE,
                                     semantic = TRUE, syn = syn),
  # Fetch duplicate sets chunk.size 2000
  t2 = ProbDup(kwic1 = GNKWIC, method = "a", excep = exep,
                                     chunksize = 2000, useBytes = TRUE,
                                     fuzzy = TRUE, phonetic = TRUE,
                                     semantic = TRUE, syn = syn),
  # Fetch duplicate sets chunk.size 100
  t3 = ProbDup(kwic1 = GNKWIC, method = "a", excep = exep,
                                     chunksize = 100, useBytes = TRUE,
                                     fuzzy = TRUE, phonetic = TRUE,
                                     semantic = TRUE, syn = syn),
  # Fetch duplicate sets useBytes = FALSE
  t4 = ProbDup(kwic1 = GNKWIC, method = "a", excep = exep,
                                     chunksize = 1000, useBytes = FALSE,
                                     fuzzy = TRUE, phonetic = TRUE,
                                     semantic = TRUE, syn = syn), times = 10)
plot(timings, col = c("#1B9E77", "#D95F02", "#7570B3", "#E7298A"),
     xlab = "Expression", ylab = "Time")
legend("topright", c("t1 : chunksize = 1000,\n     useBytes = T (default)\n",
         "t2 : chunksize = 2000,\n     useBytes = T\n",
         "t3 : chunksize = 500,\n     useBytes = T\n",
         "t4 : chunksize = 1000,\n     useBytes = F\n"),
       bty = "n", cex = 0.6)

Fig. 3. CPU time with different ProbDup arguments estimated using the microbenchmark package.

Set Review, Modification and Validation

The initially retrieved sets may be intersecting with each other because there might be accessions which occur in more than duplicate set. Disjoint sets can be generated by merging such overlapping sets using the function DisProbDup.

Disjoint sets are retrieved either individually for each type of probable duplicate sets or considering all type of sets simultaneously. In case of the latter, the disjoint of all the type of sets alone are returned in the output as an additional data frame DisjointDupicates in an object of class ProbDup.

# Load example dataset
GN <- GN1000

# Specify as a vector the database fields to be used
GNfields <- c("NationalID", "CollNo", "DonorID", "OtherID1", "OtherID2")

# Clean the data
GN[GNfields] <- lapply(GN[GNfields], function(x) DataClean(x))
y1 <- list(c("Gujarat", "Dwarf"), c("Castle", "Cary"), c("Small", "Japan"),
c("Big", "Japan"), c("Mani", "Blanco"), c("Uganda", "Erect"),
c("Mota", "Company"))
y2 <- c("Dark", "Light", "Small", "Improved", "Punjab", "SAM")
y3 <- c("Local", "Bold", "Cary", "Mutant", "Runner", "Giant", "No.",
        "Bunch", "Peanut")
GN[GNfields] <- lapply(GN[GNfields],
                       function(x) MergeKW(x, y1, delim = c("space", "dash")))
GN[GNfields] <- lapply(GN[GNfields],
                       function(x) MergePrefix(x, y2, delim = c("space", "dash")))
GN[GNfields] <- lapply(GN[GNfields],
                       function(x) MergeSuffix(x, y3, delim = c("space", "dash")))

# Generate KWIC index
GNKWIC <- KWIC(GN, GNfields)

# Specify the exceptions as a vector
exep <- c("A", "B", "BIG", "BOLD", "BUNCH", "C", "COMPANY", "CULTURE",
         "DARK", "E", "EARLY", "EC", "ERECT", "EXOTIC", "FLESH", "GROUNDNUT",
         "GUTHUKAI", "IMPROVED", "K", "KUTHUKADAL", "KUTHUKAI", "LARGE",
         "LIGHT", "LOCAL", "OF", "OVERO", "P", "PEANUT", "PURPLE", "R",
         "RED", "RUNNER", "S1", "SAM", "SMALL", "SPANISH", "TAN", "TYPE",
         "U", "VALENCIA", "VIRGINIA", "WHITE")

# Specify the synsets as a list
syn <- list(c("CHANDRA", "AH114"), c("TG1", "VIKRAM"))

# Fetch probable duplicate sets
GNdup <- ProbDup(kwic1 = GNKWIC, method = "a", excep = exep, fuzzy = TRUE,
                 phonetic = TRUE, encoding = "primary",
                 semantic = TRUE, syn = syn)
# Initial number of sets
GNdup
Method : a

KWIC1 fields : NationalID CollNo DonorID OtherID1 OtherID2
 
                   No..of.Sets     No..of.Records
FuzzyDuplicates            378                745
PhoneticDuplicates          99                260
SemanticDuplicates           2                  5
Total                      479 1010(Distinct:762)
# Get disjoint probable duplicate sets of each kind
disGNdup1 <- DisProbDup(GNdup, combine = NULL)
# # Number of sets after combining intersecting sets
disGNdup1
Method : a

KWIC1 fields : NationalID CollNo DonorID OtherID1 OtherID2
 
                   No..of.Sets     No..of.Records
FuzzyDuplicates            181                745
PhoneticDuplicates          80                260
SemanticDuplicates           2                  5
Total                      263 1010(Distinct:762)
# Get disjoint probable duplicate sets combining all the kinds of sets
disGNdup2 <- DisProbDup(GNdup, combine = c("F", "P", "S"))
# Number of sets after combining intersecting sets
disGNdup2
Method : a

KWIC1 fields : NationalID CollNo DonorID OtherID1 OtherID2
 
                  No..of.Sets    No..of.Records
DisjointDupicates         167               762
Total                     167 762(Distinct:762)

Once duplicate sets are retrieved they can be validated by manual clerical review by comparing with original PGR passport database(s) using the ReviewProbDup function. This function helps to retrieve PGR passport information associated with fuzzy, phonetic or semantic probable duplicate sets in an object of class ProbDup from the original databases(s) from which they were identified. The original information of accessions comprising a set, which have not been subjected to data standardization can be compared under manual clerical review for the validation of the set. By default only the fields(columns) which were used initially for creation of the KWIC indexes using the KWIC function are retrieved. Additional fields(columns) if necessary can be specified using the extra.db1 and extra.db2 arguments.

When any primary ID/key records in the fuzzy, phonetic or semantic duplicate sets are found to be missing from the original databases specified in db1 and db2, then they are ignored and only the matching records are considered for retrieving the information with a warning.

This may be due to data standardization of the primary ID/key field using the function DataClean before creation of the KWIC index and subsequent identification of probable duplicate sets. In such a case, it is recommended to use an identical data standardization operation on the primary ID/key field of databases specified in db1 and db2 before running this function.

With R <= v3.0.2, due to copying of named objects by list(), Invalid .internal.selfref detected and fixed... warning can appear, which may be safely ignored.

The output data frame can be subjected to clerical review either after exporting into an external spreadsheet using write.csv function or by using the edit function.

The column DEL can be used to indicate whether a record has to be deleted from a set or not. Y indicates “Yes”, and the default N indicates “No”.

The column SPLIT similarly can be used to indicate whether a record in a set has to be branched into a new set. A set of identical integers in this column other than the default 0 can be used to indicate that they are to be removed and assembled into a new set.

# Load the original database and clean the Primary ID/key field
GN1000 <- GN1000
GN1000$NationalID <- DataClean(GN1000$NationalID)

# Get the data frame for reviewing the duplicate sets identified
RevGNdup <- ReviewProbDup(pdup = disGNdup1, db1 = GN1000,
                          extra.db1 = c("SourceCountry", "TransferYear"),
                          max.count = 30, insert.blanks = TRUE)
head(RevGNdup)
  SET_NO TYPE K[a]  PRIM_ID                IDKW  DEL SPLIT COUNT K1_NationalID
1      1    F [K1] EC100277 [K1]EC100277:U44712    N     0     3      EC100277
2      1    F [K1]  EC21118  [K1]EC21118:U44712    N     0     3       EC21118
3      1    F [K1] IC494796 [K1]IC494796:U44712    N     0     3      IC494796
4     NA      <NA>     <NA>                <NA> <NA>    NA    NA          <NA>
5      1    P [K1] EC100713  [K1]EC100713:STARR    N     0    14      EC100713
6      1    P [K1] EC106985  [K1]EC106985:STARR    N     0    14      EC106985
                 K1_CollNo K1_DonorID K1_OtherID1  K1_OtherID2
1    Shulamith/ NRCG-14555   ICG-4709                 U4-47-12
2 U 4-47-12; EC 21118; UKA    ICG3265             U44712 U K A
3                U-4-47-12   ICG-6890                   U44712
4                     <NA>       <NA>        <NA>         <NA>
5               EC 100713;    ICG5296                    STARR
6                    Starr    ICG3479                         
         K1X_SourceCountry K1X_TransferYear
1                   Israel             2014
2                Australia             1989
3                  Unknown             2010
4                     <NA>               NA
5 United States of America             2004
6 United States of America             2001
# Examine and review the duplicate sets using edit function
RevGNdup <- edit(RevGNdup)

# OR examine and review the duplicate sets after exporting them as a csv file
write.csv(file="Duplicate sets for review.csv", x=RevGNdup)

After clerical review, the data frame created using the function ReviewProbDup from an object of class ProbDup can be reconstituted back to the same object after the review using the function ReconstructProbDup.

The instructions for modifying the sets entered in the appropriate format in the columns DEL and SPLIT during clerical review are taken into account for reconstituting the probable duplicate sets. Any records with Y in column DEL are deleted and records with identical integers in the column SPLIT other than the default 0 are reassembled into a new set.

# The original set data
subset(RevGNdup, SET_NO==13 & TYPE=="P", select= c(IDKW, DEL, SPLIT))
                                             IDKW DEL SPLIT
111                         [K1]EC38607:MANFREDI1   N     0
112                         [K1]EC420966:MANFREDI   N     0
113                        [K1]EC42549:MANFREDI68   N     0
114                          [K1]EC42550:MANFRED1   N     0
115 [K1]EC552714:CHAMPAQUI, [K1]EC552714:MANFREDI   N     0
116                       [K1]EC573128:MANFREDI84   N     0
117 [K1]IC304523:CHAMPAGUE, [K1]IC304523:MANFREDI   N     0
# Make dummy changes to the set for illustration
RevGNdup[c(113, 116), 6] <- "Y"
RevGNdup[c(111, 114), 7] <- 1
RevGNdup[c(112, 115, 117), 7] <- 2
# The instruction for modification in columns DEL and SPLIT
subset(RevGNdup, SET_NO==13 & TYPE=="P", select= c(IDKW, DEL, SPLIT))
                                             IDKW DEL SPLIT
111                         [K1]EC38607:MANFREDI1   N     1
112                         [K1]EC420966:MANFREDI   N     2
113                        [K1]EC42549:MANFREDI68   Y     0
114                          [K1]EC42550:MANFRED1   N     1
115 [K1]EC552714:CHAMPAQUI, [K1]EC552714:MANFREDI   N     2
116                       [K1]EC573128:MANFREDI84   Y     0
117 [K1]IC304523:CHAMPAGUE, [K1]IC304523:MANFREDI   N     2
# Reconstruct ProDup object
GNdup2 <- ReconstructProbDup(RevGNdup)
# Initial no. of sets
disGNdup1
Method : a

KWIC1 fields : NationalID CollNo DonorID OtherID1 OtherID2
 
                   No..of.Sets     No..of.Records
FuzzyDuplicates            181                745
PhoneticDuplicates          80                260
SemanticDuplicates           2                  5
Total                      263 1010(Distinct:762)
# No. of sets after modifications
GNdup2
Method : a

KWIC1 fields : NationalID CollNo DonorID OtherID1 OtherID2
 
                   No..of.Sets    No..of.Records
FuzzyDuplicates            180               523
PhoneticDuplicates          81               258
SemanticDuplicates           2                 5
Total                      263 786(Distinct:674)

Other Functions

The ProbDup object is a list of data frames of different kinds of probable duplicate sets viz- FuzzyDuplicates, PhoneticDuplicates, SemanticDuplicates and DisjointDuplicates. Each row of the component data frame will have information of a set, the type of set, the set members as well as the keywords based on which the set was formed. This data can be reshaped into long form using the function ParseProbDup. This function which will transform a ProbDup object into a single data frame.

# Convert 'ProbDup' object to a long form data frame of sets
GNdupParsed <- ParseProbDup(GNdup)
head(GNdupParsed)
  SET_NO TYPE    K  PRIM_ID                IDKW COUNT
1      1    F [K1] EC100277 [K1]EC100277:U44712     3
2      1    F [K1]  EC21118  [K1]EC21118:U44712     3
3      1    F [K1] IC494796 [K1]IC494796:U44712     3
4     NA      <NA>     <NA>                <NA>    NA
5      2    F [K1] EC100280    [K1]EC100280:NC5     3
6      2    F [K1] EC100721    [K1]EC100721:NC5     3

The prefix K* here indicates the KWIC index of origin. This is useful in ascertaining the database of origin of the accessions when method "b" or "c" was used to create the input ProbDup object.

Once the sets are reviewed and modified, the validated set data fields from the ProbDup object can be added to the original PGR passport database using the function AddProbDup. The associated data fields such as SET_NO, ID and IDKW are added based on the PRIM_ID field(column).

# Loading original database
GN2 <- GN1000

# Add the duplicates set data to the original database
GNwithdup <-  AddProbDup(pdup = GNdup, db = GN2, addto = "I")

In case more than one KWIC index was used to generate the object of class ProbDup, the argument addto can be used to specify to which database the data fields are to be added. The default "I" indicates the database from which the first KWIC index was created and "II" indicates the database from which the second index was created.

The function SplitProbDup can be used to split an object of class ProbDup into two on the basis of set counts. This is useful for reviewing separately the sets with larger set counts.

# Load PGR passport database
GN <- GN1000

# Specify as a vector the database fields to be used
GNfields <- c("NationalID", "CollNo", "DonorID", "OtherID1", "OtherID2")

# Clean the data
GN[GNfields] <- lapply(GN[GNfields], function(x) DataClean(x))
y1 <- list(c("Gujarat", "Dwarf"), c("Castle", "Cary"), c("Small", "Japan"),
c("Big", "Japan"), c("Mani", "Blanco"), c("Uganda", "Erect"),
c("Mota", "Company"))
y2 <- c("Dark", "Light", "Small", "Improved", "Punjab", "SAM")
y3 <- c("Local", "Bold", "Cary", "Mutant", "Runner", "Giant", "No.",
        "Bunch", "Peanut")
GN[GNfields] <- lapply(GN[GNfields],
                       function(x) MergeKW(x, y1, delim = c("space", "dash")))
GN[GNfields] <- lapply(GN[GNfields],
                       function(x) MergePrefix(x, y2, delim = c("space", "dash")))
GN[GNfields] <- lapply(GN[GNfields],
                       function(x) MergeSuffix(x, y3, delim = c("space", "dash")))

# Generate KWIC index
GNKWIC <- KWIC(GN, GNfields)

# Specify the exceptions as a vector
exep <- c("A", "B", "BIG", "BOLD", "BUNCH", "C", "COMPANY", "CULTURE",
         "DARK", "E", "EARLY", "EC", "ERECT", "EXOTIC", "FLESH", "GROUNDNUT",
         "GUTHUKAI", "IMPROVED", "K", "KUTHUKADAL", "KUTHUKAI", "LARGE",
         "LIGHT", "LOCAL", "OF", "OVERO", "P", "PEANUT", "PURPLE", "R",
         "RED", "RUNNER", "S1", "SAM", "SMALL", "SPANISH", "TAN", "TYPE",
         "U", "VALENCIA", "VIRGINIA", "WHITE")

# Specify the synsets as a list
syn <- list(c("CHANDRA", "AH114"), c("TG1", "VIKRAM"))

# Fetch probable duplicate sets
GNdup <- ProbDup(kwic1 = GNKWIC, method = "a", excep = exep, fuzzy = TRUE,
                 phonetic = TRUE, encoding = "primary",
                 semantic = TRUE, syn = syn)
# Split the probable duplicate sets
GNdupSplit <- SplitProbDup(GNdup, splitat = c(10, 10, 10))
GNdupSplit[[1]]
Method : a

KWIC1 fields : NationalID CollNo DonorID OtherID1 OtherID2
 
                   No..of.Sets     No..of.Records
FuzzyDuplicates            338                744
PhoneticDuplicates          99                260
SemanticDuplicates           2                  5
Total                      439 1009(Distinct:762)
GNdupSplit[[3]]
Method : a

KWIC1 fields : NationalID CollNo DonorID OtherID1 OtherID2
 
                No..of.Sets    No..of.Records
FuzzyDuplicates          40               136
Total                    40 136(Distinct:136)

Alternatively, two different ProbDup objects can be merged together using the function MergeProbDup.

GNdupMerged <- MergeProbDup(GNdupSplit[[1]], GNdupSplit[[3]])
GNdupMerged
Method : a

KWIC1 fields : NationalID CollNo DonorID OtherID1 OtherID2
 
                   No..of.Sets     No..of.Records
FuzzyDuplicates            378                745
PhoneticDuplicates          99                260
SemanticDuplicates           2                  5
Total                      479 1010(Distinct:762)

The summary of accessions according to a grouping factor field(column) in the original database(s) within the probable duplicate sets retrieved in a ProbDup object can be visualized by the ViewProbDup function. The resulting plot can be used to examine the extent of probable duplication within and between groups of accessions records.

# Load PGR passport databases
GN1 <- GN1000[!grepl("^ICG", GN1000$DonorID), ]
GN1$DonorID <- NULL
GN2 <- GN1000[grepl("^ICG", GN1000$DonorID), ]
GN2 <- GN2[!grepl("S", GN2$DonorID), ]
GN2$NationalID <- NULL

GN1$SourceCountry <- toupper(GN1$SourceCountry)
GN2$SourceCountry <- toupper(GN2$SourceCountry)

GN1$SourceCountry <- gsub("UNITED STATES OF AMERICA", "USA", GN1$SourceCountry)
GN2$SourceCountry <- gsub("UNITED STATES OF AMERICA", "USA", GN2$SourceCountry)

# Specify as a vector the database fields to be used
GN1fields <- c("NationalID", "CollNo", "OtherID1", "OtherID2")
GN2fields <- c("DonorID", "CollNo", "OtherID1", "OtherID2")

# Clean the data
GN1[GN1fields] <- lapply(GN1[GN1fields], function(x) DataClean(x))
GN2[GN2fields] <- lapply(GN2[GN2fields], function(x) DataClean(x))
y1 <- list(c("Gujarat", "Dwarf"), c("Castle", "Cary"), c("Small", "Japan"),
           c("Big", "Japan"), c("Mani", "Blanco"), c("Uganda", "Erect"),
           c("Mota", "Company"))
y2 <- c("Dark", "Light", "Small", "Improved", "Punjab", "SAM")
y3 <- c("Local", "Bold", "Cary", "Mutant", "Runner", "Giant", "No.",
        "Bunch", "Peanut")
GN1[GN1fields] <- lapply(GN1[GN1fields],
                         function(x) MergeKW(x, y1, delim = c("space", "dash")))
GN1[GN1fields] <- lapply(GN1[GN1fields],
                         function(x) MergePrefix(x, y2, delim = c("space", "dash")))
GN1[GN1fields] <- lapply(GN1[GN1fields],
                         function(x) MergeSuffix(x, y3, delim = c("space", "dash")))
GN2[GN2fields] <- lapply(GN2[GN2fields],
                         function(x) MergeKW(x, y1, delim = c("space", "dash")))
GN2[GN2fields] <- lapply(GN2[GN2fields],
                         function(x) MergePrefix(x, y2, delim = c("space", "dash")))
GN2[GN2fields] <- lapply(GN2[GN2fields],
                         function(x) MergeSuffix(x, y3, delim = c("space", "dash")))

# Remove duplicated DonorID records in GN2
GN2 <- GN2[!duplicated(GN2$DonorID), ]

# Generate KWIC index
GN1KWIC <- KWIC(GN1, GN1fields)
GN2KWIC <- KWIC(GN2, GN2fields)

# Specify the exceptions as a vector
exep <- c("A", "B", "BIG", "BOLD", "BUNCH", "C", "COMPANY", "CULTURE",
          "DARK", "E", "EARLY", "EC", "ERECT", "EXOTIC", "FLESH", "GROUNDNUT",
          "GUTHUKAI", "IMPROVED", "K", "KUTHUKADAL", "KUTHUKAI", "LARGE",
          "LIGHT", "LOCAL", "OF", "OVERO", "P", "PEANUT", "PURPLE", "R",
          "RED", "RUNNER", "S1", "SAM", "SMALL", "SPANISH", "TAN", "TYPE",
          "U", "VALENCIA", "VIRGINIA", "WHITE")

# Specify the synsets as a list
syn <- list(c("CHANDRA", "AH114"), c("TG1", "VIKRAM"))
GNdupc <- ProbDup(kwic1 = GN1KWIC, kwic2 = GN2KWIC, method = "c",
                  excep = exep, fuzzy = TRUE, phonetic = TRUE,
                  encoding = "primary", semantic = TRUE, syn = syn)
Fuzzy matching

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Phonetic matching

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Semantic matching

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# Get the summary data.frames and Grob
GNdupcView <- ViewProbDup(GNdupc, GN1, GN2, "SourceCountry", "SourceCountry",
                         max.count = 30, select = c("INDIA", "USA"), order = "type",
                         main = "Groundnut Probable Duplicates")
Warning: 
[1m
[22mThe `<scale>` argument of `guides()` cannot be `FALSE`. Use "none" instead as
of ggplot2 3.3.4.

[36mℹ
[39m The deprecated feature was likely used in the 
[34mPGRdup
[39m package.
  Please report the issue at 
[3m
[34m<https://github.com/aravind-j/PGRdup/issues>
[39m
[23m.
This warning is displayed once every 8 hours.
Call `lifecycle::last_lifecycle_warnings()` to see where this warning was
generated.
# View the summary data.frames
GNdupcView[[1]]
GNdupcView[[2]]
# Plot the summary visualization
library(gridExtra)
grid.arrange(GNdupcView[[3]])

Fig. 5. Summary visualization of groundnut probable duplicate sets retrieved according to SourceCountry field.

The function KWCounts can be used to compute the keyword counts from PGR passport database fields(columns) which are considered for identification of probable duplicates. These keyword counts can give a rough indication of the completeness of the data in such fields (Fig. 3).

# Compute the keyword counts for the whole data
GNKWCouts <- KWCounts(GN, GNfields, exep)

# Compute the keyword counts for 'duplicated' records
GND <- ParseProbDup(disGNdup2, Inf, F)$PRIM_ID

GNDKWCouts <- KWCounts(GN[GN$NationalID %in% GND, ],
                       GNfields, exep)

# Compute the keyword counts for 'unique' records
GNUKWCouts <- KWCounts(GN[!GN$NationalID %in% GND, ],
                       GNfields, exep)

# Plot the counts as barplot
par(mfrow = c(3,1))

bp1 <- barplot(table(GNKWCouts$COUNT),
               xlab = "Word count", ylab = "Frequency",
               main = "A", col = "#1B9E77")
text(bp1, 0, table(GNKWCouts$COUNT),cex = 1, pos = 3)
legend("topright", paste("No. of records =",
                         nrow(GN)),
       bty = "n")

bp2 <- barplot(table(GNDKWCouts$COUNT),
               xlab = "Word count", ylab = "Frequency",
               main = "B", col = "#D95F02")
text(bp2, 0, table(GNDKWCouts$COUNT),cex = 1, pos = 3)
legend("topright", paste("No. of records =",
                   nrow(GN[GN$NationalID %in% GND, ])),
       bty = "n")

bp3 <- barplot(table(GNUKWCouts$COUNT),
               xlab = "Word count", ylab = "Frequency",
               main = "C", col = "#7570B3")
text(bp3, 0, table(GNUKWCouts$COUNT),cex = 1, pos = 3)
legend("topright", paste("No. of records =",
                   nrow(GN[!GN$NationalID %in% GND, ])),
       bty = "n")

Fig. 6. The keyword counts in the database fields considered for identification of probable duplicates for A. the entire GN1000 dataset, B. the probable duplicate records alone and C. the unique records alone.

Citing PGRdup

citation("PGRdup")
To cite the R package 'PGRdup' in publications use:

  Aravind, J., Radhamani, J., Kalyani Srinivasan, Ananda Subhash, B.,
  and Tyagi, R. K.  ().  PGRdup: Discover Probable Duplicates in Plant
  Genetic Resources Collections. R package version 0.2.3.9,
  https://github.com/aravind-j/PGRdup,https://cran.r-project.org/package=PGRdup.

A BibTeX entry for LaTeX users is

  @Manual{,
    title = {PGRdup: Discover Probable Duplicates in Plant Genetic Resources Collections},
    author = {J. Aravind and J. Radhamani and {Kalyani Srinivasan} and B. {Ananda Subhash} and Rishi Kumar Tyagi},
    note = {R package version 0.2.3.9 https://github.com/aravind-j/PGRdup, https://cran.r-project.org/package=PGRdup},
  }

This free and open-source software implements academic research by the
authors and co-workers. If you use it, please support the project by
citing the package.

Session Info

R version 4.3.1 (2023-06-16)
Platform: x86_64-apple-darwin20 (64-bit)
Running under: macOS Monterey 12.6.7

Matrix products: default
BLAS:   /Library/Frameworks/R.framework/Versions/4.3-x86_64/Resources/lib/libRblas.0.dylib 
LAPACK: /Library/Frameworks/R.framework/Versions/4.3-x86_64/Resources/lib/libRlapack.dylib;  LAPACK version 3.11.0

locale:
[1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8

time zone: UTC
tzcode source: internal

attached base packages:
[1] stats     graphics  grDevices utils     datasets  methods   base     

other attached packages:
[1] PGRdup_0.2.3.9     gridExtra_2.3      wordcloud_2.6      RColorBrewer_1.1-3
[5] diagram_1.6.5      shape_1.4.6       

loaded via a namespace (and not attached):
 [1] sass_0.4.7            utf8_1.2.3            bitops_1.0-7         
 [4] stringi_1.7.12        digest_0.6.33         magrittr_2.0.3       
 [7] evaluate_0.21         grid_4.3.1            fastmap_1.1.1        
[10] rprojroot_2.0.3       jsonlite_1.8.7        httr_1.4.7           
[13] purrr_1.0.2           fansi_1.0.4           scales_1.2.1         
[16] stringdist_0.9.10     XML_3.99-0.14         microbenchmark_1.4.10
[19] textshaping_0.3.6     jquerylib_0.1.4       cli_3.6.1            
[22] rlang_1.1.1           munsell_0.5.0         withr_2.5.0          
[25] cachem_1.0.8          yaml_2.3.7            tools_4.3.1          
[28] parallel_4.3.1        memoise_2.0.1         colorspace_2.1-0     
[31] ggplot2_3.4.3         curl_5.0.2            vctrs_0.6.3          
[34] R6_2.5.1              lifecycle_1.0.3       stringr_1.5.0        
[37] fs_1.6.3              ragg_1.2.5            pkgconfig_2.0.3      
[40] desc_1.4.2            pkgdown_2.0.7         bslib_0.5.1          
[43] pillar_1.9.0          gtable_0.3.4          Rcpp_1.0.11          
[46] data.table_1.14.8     glue_1.6.2            systemfonts_1.0.4    
[49] xfun_0.40             tibble_3.2.1          highr_0.10           
[52] knitr_1.43            farver_2.1.1          htmltools_0.5.6      
[55] igraph_1.5.1          labeling_0.4.3        rmarkdown_2.24       
[58] compiler_4.3.1        RCurl_1.98-1.12      

References

Knüpffer, H. 1988. “The European Barley Database of the ECP/GR: An Introduction.” Die Kulturpflanze 36 (1): 135–62. https://doi.org/10.1007/BF01986957.
Knüpffer, H., L. Frese, and M. W. M. Jongen. 1997. “Using Central Crop Databases: Searching for Duplicates and Gaps.” In Central Crop Databases: Tools for Plant Genetic Resources Management. Report of a Workshop, Budapest, Hungary, 13-16 October 1996, edited by E. Lipman, M. W. M. Jongen, T. J. L. van Hintum, T. Gass, and L. Maggioni, 67–77. Rome, Italy and Wageningen, The Netherlands: International Plant Genetic Resources Institute and Centre for Genetic Resources. https://www.bioversityinternational.org/index.php?id=244&tx_news_pi1%5Bnews%5D=334&cHash=3738ae238a450ff71bb1cb087687ac9c.
Loo, M. P. J. van der. 2014. “The Stringdist Package for Approximate String Matching.” R Journal 6 (1): 111–22. https://journal.r-project.org/archive/2014/RJ-2014-011/index.html.
Philips, Lawrence. 2000. “The Double Metaphone Search Algorithm.” C/C++ Users Journal 18 (6): 38–43. https://dl.acm.org/citation.cfm?id=349124.349132.